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High Sensitive ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)
CC-HEA551Hu
New product
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Data sheet
| Size | 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100 |
| Storage Conditions | -20°C |
| Shipping Conditions | Entre 4°C y 8°C |
| Applications | Enzyme-linked immunosorbent assay for Antigen Detection. |
| Detection Range | 2.47-200pg/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 0.93pg/mL |
| Research Area | Cytokine |
| Organism species | Homo sapiens (Human) |
| Alternative Names | B-FGF |
| Item Name | Fibroblast Growth Factor 2, Basic |
| Assay Length | 3h |
| Method | Competitive Inhibition |
| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Formato | 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100 |
| Test Principle | This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to High Sensitive Fibroblast Growth Factor 2, Basic (FGF2) has been pre-coated onto a microplate. A competitive inhibition reaction is launched betw |
| Assay procedure summary | 1. Prepare all reagents, samples and standards |
| UniProt ID | P09038 |
More info
This assay has high sensitivity and excellent specificity for detection of High Sensitive Fibroblast Growth Factor 2, Basic (FGF2).
No significant cross-reactivity or interference between High Sensitive Fibroblast Growth Factor 2, Basic (FGF2) and analogues was observed.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Fibroblast Growth Factor 2, Basic (FGF2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Fibroblast Growth Factor 2, Basic (FGF2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.