High Sensitive ELISA Kit for Stromal Cell Derived Factor 1 (SDF1) View larger

High Sensitive ELISA Kit for Stromal Cell Derived Factor 1 (SDF1)

CC-HEA122Ra

New product

48T, 96T, 96T×5, 96T×10, 96T×100

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Data sheet

Size 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100
Storage Conditions -20°C
Shipping Conditions Entre 4°C y 8°C
Applications Enzyme-linked immunosorbent assay for Antigen Detection.
Detection Range 15.6-1,000pg/mL
Sensitivity The minimum detectable dose of this kit is typically less than 6.7pg/mL
Research Area Cytokine
Organism species Rattus norvegicus (Rat)
Alternative Names CXCL12
Item Name Stromal Cell Derived Factor 1
Assay Length 3h
Method Double-antibody Sandwich
Sample Type serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Formato 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100
Test Principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Stromal Cell Derived Factor 1 (SDF1). Standards or samples are then added to t
Assay procedure summary 1. Prepare all reagents, samples and standards
UniProt ID Q9QZD1

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Especificidad:

This assay has high sensitivity and excellent specificity for detection of High Sensitive Stromal Cell Derived Factor 1 (SDF1).
No significant cross-reactivity or interference between High Sensitive Stromal Cell Derived Factor 1 (SDF1) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Stromal Cell Derived Factor 1 (SDF1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Stromal Cell Derived Factor 1 (SDF1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Estabilidad:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

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