Wide-range ELISA Kit for Glycated Hemoglobin A1c (HbA1c) View larger

Wide-range ELISA Kit for Glycated Hemoglobin A1c (HbA1c)

CC-WEA190Mu

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48T, 96T, 96T×5, 96T×10, 96T×100

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Data sheet

Size 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100
Storage Conditions -20°C
Shipping Conditions Entre 4°C y 8°C
Applications Enzyme-linked immunosorbent assay for Antigen Detection.
Detection Range 12.35-1000ug/mL
Sensitivity The minimum detectable dose of this kit is typically less than
Organism species Mus musculus (Mouse)
Alternative Names Glycosylated Hemoglobin
Item Name Glycated Hemoglobin A1c
Assay Length 3h
Method Competitive Inhibition
Sample Type plasma and erythrocyte lysates
Formato 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100
Test Principle This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Wide-range Glycated Hemoglobin A1c (HbA1c) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin la
Assay procedure summary 1. Prepare all reagents, samples and standards
UniProt ID -

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Especificidad:

This assay has high sensitivity and excellent specificity for detection of Wide-range Glycated Hemoglobin A1c (HbA1c).
No significant cross-reactivity or interference between Wide-range Glycated Hemoglobin A1c (HbA1c) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range Glycated Hemoglobin A1c (HbA1c) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Glycated Hemoglobin A1c (HbA1c) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Estabilidad:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

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