ELISA Kit for Tetraspanin 30Cluster of Differentiation 63 (CD63) View larger

ELISA Kit for Tetraspanin 30Cluster of Differentiation 63 (CD63)

CC-SEB345Hu

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48T, 96T, 96T×5, 96T×10, 96T×100

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Data sheet

Size 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100
Storage Conditions -20°C
Shipping Conditions Entre 4°C y 8°C
Applications Enzyme-linked immunosorbent assay for Antigen Detection.
Detection Range 0.156-10ng/mL
Sensitivity The minimum detectable dose of this kit is typically less than 0.061ng/mL
Research Area Signal transduction
Organism species Homo sapiens (Human)
Alternative Names TSPAN30
Item Name Tetraspanin 30Cluster of Differentiation 63
Assay Length 3h
Method Double-antibody Sandwich
Sample Type serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Formato 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100
Test Principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tetraspanin 30Cluster of Differentiation 63 (CD63). Standards or samples are then added to th
Assay procedure summary 1. Prepare all reagents, samples and standards
UniProt ID P08962

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Especificidad:

This assay has high sensitivity and excellent specificity for detection of Tetraspanin 30Cluster of Differentiation 63 (CD63).
No significant cross-reactivity or interference between Tetraspanin 30Cluster of Differentiation 63 (CD63) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tetraspanin 30Cluster of Differentiation 63 (CD63) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tetraspanin 30Cluster of Differentiation 63 (CD63) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Estabilidad:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

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