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nNOS:N-Terminal (neuronal Nitric Oxide Synthase) Antibody
IM-24431-100 µl
New product
Whole Serum
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Data sheet
| Size | 100 µl |
| Applications | Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot |
| Target | Human nNOS (134-148) |
| Type Clonality | polyclonal |
| Immunogen | Human nNOS (134-148) |
| Formato | Whole Serum |
| Host | Rabbit |
| Reactivity | Mouse, Rat |
| Conjugation | Unconjugated |
More info
The ImmunoStar N-terminal neuronal nitric oxide synthase antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus, striatum, cortex and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/1,000 - 1/2,000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP. By Western blot analysis of brain homogenates the antibody specifically labels a band of approximately 155 kD. Immunolabeling is completely abolished by pre-adsorption with synthetic human nNOS (134-148) at 5 µg per mL of diluted antibody. No cross reactivity with other forms of NOS was observed.