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ELISA Kit for Vimentin (VIM)
CC-SEB040Bo
New product
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Data sheet
| Size | 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100 |
| Applications | Enzyme-linked immunosorbent assay for Antigen Detection. |
| Detection Range | 7.8-500ng/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 2.7ng/mL |
| Research Area | Tumor immunity |
| Organism species | Bos taurus |
| Item Name | Vimentin |
| Assay Length | 3h |
| Method | Double-antibody Sandwich |
| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vimentin (VIM). Standards or samples are then added to the appropriate microtiter plate wells |
| Assay procedure summary | 1. Prepare all reagents, samples and standards |
| UniProt ID | - |
More info
This assay has high sensitivity and excellent specificity for detection of Vimentin (VIM).
No significant cross-reactivity or interference between Vimentin (VIM) and analogues was observed.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vimentin (VIM) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vimentin (VIM) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.