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ELISA Kit for RUN Domain And Cysteine Rich Domain Containing, Beclin 1 Interacting Protein (Rubicon)
CC-SEM905Hu
New product
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Data sheet
| Size | 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100 |
| Applications | Enzyme-linked immunosorbent assay for Antigen Detection. |
| Detection Range | 0.312-20ng/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 0.124ng/mL |
| Research Area | Signal transduction |
| Organism species | Homo sapiens (Human) |
| Alternative Names | Baron |
| Item Name | RUN Domain And Cysteine Rich Domain Containing, Beclin 1 Interacting Protein |
| Assay Length | 3h |
| Method | Double-antibody Sandwich |
| Sample Type | Tissue homogenates, cell lysates and other biological fluids |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RUN Domain And Cysteine Rich Domain Containing, Beclin 1 Interacting Protein (Rubicon). Stand |
| Assay procedure summary | 1. Prepare all reagents, samples and standards |
| UniProt ID | - |
More info
This assay has high sensitivity and excellent specificity for detection of RUN Domain And Cysteine Rich Domain Containing, Beclin 1 Interacting Protein (Rubicon).
No significant cross-reactivity or interference between RUN Domain And Cysteine Rich Domain Containing, Beclin 1 Interacting Protein (Rubicon) and analogues was observed.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RUN Domain And Cysteine Rich Domain Containing, Beclin 1 Interacting Protein (Rubicon) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RUN Domain And Cysteine Rich Domain Containing, Beclin 1 Interacting Protein (Rubicon) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.