New ELISA Kit for Matrix Metalloproteinase 9 (MMP9) View larger

ELISA Kit for Matrix Metalloproteinase 9 (MMP9)

CC-SEA553Si

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48T, 96T, 96T×5, 96T×10, 96T×100

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Data sheet

Size 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100
Applications Enzyme-linked immunosorbent assay for Antigen Detection.
Detection Range 0.156-10ng/mL
Sensitivity The minimum detectable dose of this kit is typically less than 0.064ng/mL
Research Area Enzyme & Kinase
Organism species Rhesus monkey (Simian)
Alternative Names GELB
Item Name Matrix Metalloproteinase 9
Assay Length 3h
Method Double-antibody Sandwich
Sample Type Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 9 (MMP9). Standards or samples are then added to the appropriate mic
Assay procedure summary 1. Prepare all reagents, samples and standards
UniProt ID -

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Especificidad:

This assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 9 (MMP9).
No significant cross-reactivity or interference between Matrix Metalloproteinase 9 (MMP9) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 9 (MMP9) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 9 (MMP9) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Estabilidad:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

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