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ELISA Kit for Hepcidin (Hepc)
CC-SEB979Mu
New product
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Data sheet
| Size | 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100 |
| Applications | Enzyme-linked immunosorbent assay for Antigen Detection. |
| Detection Range | 62.5-4,000pg/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 26.3pg/mL |
| Research Area | Metabolic pathway |
| Organism species | Mus musculus (Mouse) |
| Alternative Names | HAMP |
| Item Name | Hepcidin |
| Assay Length | 3h |
| Method | Double-antibody Sandwich |
| Sample Type | serum, plasma, urine and other biological fluids |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hepcidin (Hepc). Standards or samples are then added to the appropriate microtiter plate well |
| Assay procedure summary | 1. Prepare all reagents, samples and standards |
| UniProt ID | Q9EQ21 |
More info
This assay has high sensitivity and excellent specificity for detection of Hepcidin (Hepc).
No significant cross-reactivity or interference between Hepcidin (Hepc) and analogues was observed.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hepcidin (Hepc) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hepcidin (Hepc) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.