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ELISA Kit for Collagen Type I (COL1)
CC-SEA571Rb
New product
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Data sheet
| Size | 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100 |
| Applications | Enzyme-linked immunosorbent assay for Antigen Detection. |
| Detection Range | 31.2-2,000pg/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 12.4pg/mL |
| Research Area | Metabolic pathway |
| Organism species | Oryctolagus cuniculus (Rabbit) |
| Item Name | Collagen Type I |
| Assay Length | 3h |
| Method | Double-antibody Sandwich |
| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type I (COL1). Standards or samples are then added to the appropriate microtiter pla |
| Assay procedure summary | 1. Prepare all reagents, samples and standards |
| UniProt ID | - |
More info
This assay has high sensitivity and excellent specificity for detection of Collagen Type I (COL1).
No significant cross-reactivity or interference between Collagen Type I (COL1) and analogues was observed.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type I (COL1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type I (COL1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.