New ELISA Kit for Arginine Decarboxylase (ADC) View larger

ELISA Kit for Arginine Decarboxylase (ADC)

CC-SEG460Mu

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48T, 96T, 96T×5, 96T×10, 96T×100

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Data sheet

Size 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100
Applications Enzyme-linked immunosorbent assay for Antigen Detection.
Detection Range 0.156-10ng/mL
Sensitivity The minimum detectable dose of this kit is typically less than 0.055ng/mL
Research Area Enzyme & Kinase
Organism species Mus musculus (Mouse)
Alternative Names AZIN2
Item Name Arginine Decarboxylase
Assay Length 3h
Method Double-antibody Sandwich
Sample Type tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arginine Decarboxylase (ADC). Standards or samples are then added to the appropriate microtit
Assay procedure summary 1. Prepare all reagents, samples and standards
UniProt ID Q8BVM4

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Especificidad:

This assay has high sensitivity and excellent specificity for detection of Arginine Decarboxylase (ADC).
No significant cross-reactivity or interference between Arginine Decarboxylase (ADC) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Arginine Decarboxylase (ADC) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Arginine Decarboxylase (ADC) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Estabilidad:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

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