New ELISA Kit for Apelin 36 (AP36) View larger

ELISA Kit for Apelin 36 (AP36)

CC-CEB865Ra

New product

48T, 96T, 96T×5, 96T×10, 96T×100

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Data sheet

Size 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100
Applications Enzyme-linked immunosorbent assay for Antigen Detection.
Detection Range 7.41-600pg/mL
Sensitivity The minimum detectable dose of this kit is typically less than 2.84pg/mL
Research Area Metabolic pathway
Organism species Rattus norvegicus (Rat)
Alternative Names APLN36
Item Name Apelin 36
Assay Length 2h
Method Competitive Inhibition
Sample Type serum, plasma and other biological fluids
Test Principle This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Apelin 36 (AP36) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Apelin 36 (AP36) and
Assay procedure summary 1. Prepare all reagents, samples and standards
UniProt ID -

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Especificidad:

This assay has high sensitivity and excellent specificity for detection of Apelin 36 (AP36).
No significant cross-reactivity or interference between Apelin 36 (AP36) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apelin 36 (AP36) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apelin 36 (AP36) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Estabilidad:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

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