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ELISA Kit for Anti-Cytochrome P450 2E1 Antibody (Anti-CYP2E1)
CC-AEA988Hu
New product
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Data sheet
| Size | 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100 |
| Applications | Enzyme-linked immunosorbent assay for Antibody Detection. |
| Detection Range | 1.56-100ng/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 0.55ng/mL |
| Research Area | Signal transduction |
| Organism species | Homo sapiens (Human) |
| Alternative Names | CPE1 |
| Item Name | Cytochrome P450 2E1 |
| Assay Length | 2h, 30min |
| Method | Competitive Inhibition |
| Sample Type | serum, plasma and other biological fluids |
| Test Principle | The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is |
| Assay procedure summary | 1. Prepare all reagents, samples and standards |
| UniProt ID | P05181 |
More info
This assay has high sensitivity and excellent specificity for detection of Anti-Cytochrome P450 2E1 Antibody (Anti-CYP2E1).
No significant cross-reactivity or interference between Anti-Cytochrome P450 2E1 Antibody (Anti-CYP2E1) and analogues was observed.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Cytochrome P450 2E1 Antibody (Anti-CYP2E1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Cytochrome P450 2E1 Antibody (Anti-CYP2E1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.