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ELISA Kit for Immunoglobulin G2 (IgG2)
CC-CEB682Bo
New product
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Data sheet
| Size | 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100 |
| Applications | Enzyme-linked immunosorbent assay for Antigen Detection. |
| Detection Range | 246.9-20,000ng/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 97.3ng/mL |
| Research Area | Infection immunity |
| Organism species | Bos taurus |
| Alternative Names | IGHG2 |
| Item Name | Immunoglobulin G2 |
| Assay Length | 2h |
| Method | Competitive Inhibition |
| Sample Type | serum, plasma and other biological fluids |
| Formato | 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100 |
| Test Principle | This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to Immunoglobulin G2 (IgG2) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between Horseradish Peroxidase (HRP) labeled |
| Assay procedure summary | 1. Prepare all reagents, samples and standards |
| UniProt ID | - |
More info
This assay has high sensitivity and excellent specificity for detection of Immunoglobulin G2 (IgG2).
No significant cross-reactivity or interference between Immunoglobulin G2 (IgG2) and analogues was observed.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Immunoglobulin G2 (IgG2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Immunoglobulin G2 (IgG2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.