ELISA Kit for Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3) View larger

ELISA Kit for Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3)

CC-SEH136Mu

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48T, 96T, 96T×5, 96T×10, 96T×100

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Data sheet

Size 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100
Storage Conditions -20°C
Shipping Conditions Entre 4°C y 8°C
Applications Enzyme-linked immunosorbent assay for Antigen Detection.
Detection Range 0.156-10ng/mL
Sensitivity The minimum detectable dose of this kit is typically less than 0.058ng/mL
Research Area Enzyme & Kinase
Organism species Mus musculus (Mouse)
Alternative Names SPTLC2L
Item Name Serine Palmitoyltransferase, Long Chain Base Subunit 3
Assay Length 3h
Method Double-antibody Sandwich
Sample Type Tissue homogenates, cell lysates and other biological fluids.
Formato 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100
Test Principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3). Standards or samples are the
Assay procedure summary 1. Prepare all reagents, samples and standards
UniProt ID Q8BG54

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Especificidad:

This assay has high sensitivity and excellent specificity for detection of Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3).
No significant cross-reactivity or interference between Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Estabilidad:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

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