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ELISA Kit for Triiodothyronine (T3)
CC-CEA453Ge
New product
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Data sheet
| Size | 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100 |
| Storage Conditions | -20°C |
| Shipping Conditions | Entre 4°C y 8°C |
| Applications | Enzyme-linked immunosorbent assay for Antigen Detection. |
| Detection Range | 123.5-10,000pg/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 51.4pg/mL |
| Research Area | Endocrinology |
| Organism species | Pan-species (General) |
| Alternative Names | 3,3',5-Triiodo-L-Thyronine |
| Item Name | Triiodothyronine |
| Assay Length | 2h |
| Method | Competitive Inhibition |
| Sample Type | serum, plasma and other biological fluids |
| Formato | 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100 |
| Test Principle | This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Triiodothyronine (T3) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Triiodothyronin |
| Assay procedure summary | 1. Prepare all reagents, samples and standards |
| UniProt ID | - |
More info
This assay has high sensitivity and excellent specificity for detection of Triiodothyronine (T3).
No significant cross-reactivity or interference between Triiodothyronine (T3) and analogues was observed.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Triiodothyronine (T3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Triiodothyronine (T3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.