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In addition to 5'-3' DNA polymerase activity, it also possesses 3'-5' exonuclease (proofreading) activity. <i>Pfu</i> DNA Polyme
AB-D0014
New product
Pfu DNA Polymerase
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Data sheet
| Size | 2500 Units |
| Storage Conditions | Store at -20ºC. Guaranteed stable for 6 months when properly stored. |
| Supplied Product | 10x Pfu buffer<br>10x Pfu Buffer with MgSO4<br>25 mM MgSO4 |
| Quality control testing | Free of detectable, non-specific nucleases. |
| Storage Buffer | 20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% Nonidet P40, 0.5% Tween 20 and 50% glycerol. |
More info
In addition to 5'-3' DNA polymerase activity, it also possesses 3'-5' exonuclease (proofreading) activity. Pfu DNA Polymerase exhibits the lowest error rate of any thermostable DNA polymerase studied, and is even up to ten-fold more accurate than normal Taq DNA polymerase. Consequently, Pfu DNA Polymerase is useful for polymerization reactions requiring high-fidelity synthesis.