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CLIA Kit for Fibroblast Growth Factor 15 (FGF15)
CC-CCL154Mu
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Data sheet
| Size | 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100 |
| Applications | Chemiluminescent immunoassay for Antigen Detection. |
| Detection Range | 3.9-1,000pg/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 1.7pg/mL |
| Research Area | Human gene deletion |
| Organism species | Mus musculus (Mouse) |
| Item Name | Fibroblast Growth Factor 15 |
| Assay Length | 2h |
| Method | Competitive Inhibition |
| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Test Principle | The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Fibroblast Growth Factor 15 (FGF15). A competitive inhibition reaction is launched between biotin labeled Fibroblast Growth Factor 15 (FGF15) and unlabeled Fibr |
| Assay procedure summary | 1. Prepare all reagents, samples and standards |
| UniProt ID | - |
More info
This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 15 (FGF15).
No significant cross-reactivity or interference between Fibroblast Growth Factor 15 (FGF15) and analogues was observed.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 15 (FGF15) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 15 (FGF15) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.