CC-CCV808Ge
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Size | 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100 |
Storage Conditions | -20°C |
Applications | Chemiluminescent immunoassay for Antigen Detection. |
Detection Range | 39.06-10,000ng/mL |
Sensitivity | The minimum detectable dose of this kit is typically less than 14.17ng/mL |
Organism species | Pan-species (General) |
Alternative Names | CP |
Item Name | Phosphocreatine |
Assay Length | 2h |
Method | Competitive Inhibition |
Sample Type | serum, plasma and other biological fluids |
Formato | 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100 |
Test Principle | The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Phosphocreatine (PCr). A competitive inhibition reaction is launched between biotin labeled Phosphocreatine (PCr) and unlabeled Phosphocreatine (PCr) (Standards |
Assay procedure summary | 1. Prepare all reagents, samples and standards |
UniProt ID | - |
This assay has high sensitivity and excellent specificity for detection of Phosphocreatine (PCr).
No significant cross-reactivity or interference between Phosphocreatine (PCr) and analogues was observed.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phosphocreatine (PCr) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phosphocreatine (PCr) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.