CLIA Kit for Ionized Calcium-binding Adapter Molecule 1 (IBA1) View larger

CLIA Kit for Ionized Calcium-binding Adapter Molecule 1 (IBA1)

CC-SCC288Ra

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48T, 96T, 96T×5, 96T×10, 96T×100

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Data sheet

Size 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100
Storage Conditions -20°C
Shipping Conditions Entre 4°C y 8°C
Applications Chemiluminescent immunoassay for Antigen Detection.
Detection Range 2.74-2,000pg/mL
Sensitivity The minimum detectable dose of this kit is typically less than 0.97pg/mL
Research Area Cytokine
Organism species Rattus norvegicus (Rat)
Alternative Names AIF1
Item Name Ionized Calcium-binding Adapter Molecule 1
Assay Length 2h, 40min
Method Double-antibody Sandwich
Sample Type Tissue homogenates, cell lysates and other biological fluids
Formato 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100
Test Principle The microplate provided in this kit has been pre-coated with an antibody specific to Ionized Calcium-binding Adapter Molecule 1 (IBA1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to I
Assay procedure summary 1. Prepare all reagents, samples and standards
UniProt ID P55009

More info

Especificidad:

This assay has high sensitivity and excellent specificity for detection of Ionized Calcium-binding Adapter Molecule 1 (IBA1).
No significant cross-reactivity or interference between Ionized Calcium-binding Adapter Molecule 1 (IBA1) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ionized Calcium-binding Adapter Molecule 1 (IBA1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ionized Calcium-binding Adapter Molecule 1 (IBA1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Estabilidad:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

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