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CLIA Kit for Nuclear Factor Kappa B (NFkB)
CC-SCB824Ra
New product
Ao comprar este produto pode ganhar até 9 pontos de fidelização. Seu carrinho totalizará 9 pontos de fidelização que podem ser convertidos num vale de desconto de 36.00EUR.
Data sheet
| Size | 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100 |
| Storage Conditions | -20°C |
| Shipping Conditions | Entre 4°C y 8°C |
| Applications | Chemiluminescent immunoassay for Antigen Detection. |
| Detection Range | 27.4-20,000pg/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 12.5pg/mL |
| Research Area | Signal transduction |
| Organism species | Rattus norvegicus (Rat) |
| Alternative Names | NF-KB1 |
| Item Name | Nuclear Factor Kappa B |
| Assay Length | 2h, 40min |
| Method | Double-antibody Sandwich |
| Sample Type | Tissue homogenates, cell lysates and other biological fluids |
| Formato | 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100 |
| Test Principle | The microplate provided in this kit has been pre-coated with an antibody specific to Nuclear Factor Kappa B (NFkB). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Nuclear Factor Kappa |
| Assay procedure summary | 1. Prepare all reagents, samples and standards |
| UniProt ID | Q63369 |
More info
This assay has high sensitivity and excellent specificity for detection of Nuclear Factor Kappa B (NFkB).
No significant cross-reactivity or interference between Nuclear Factor Kappa B (NFkB) and analogues was observed.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nuclear Factor Kappa B (NFkB) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nuclear Factor Kappa B (NFkB) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.