CLIA Kit for Ghrelin (GHRL) View larger

CLIA Kit for Ghrelin (GHRL)

CC-CCA991Ga

New product

48T, 96T, 96T×5, 96T×10, 96T×100

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Data sheet

Size 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100
Storage Conditions -20°C
Shipping Conditions Entre 4°C y 8°C
Applications Chemiluminescent immunoassay for Antigen Detection.
Detection Range 39.06-10,000pg/mL
Sensitivity The minimum detectable dose of this kit is typically less than 17.31pg/mL
Research Area Signal transduction
Organism species Chicken (Gallus)
Alternative Names MTLRP
Item Name Ghrelin
Assay Length 2h
Method Competitive Inhibition
Sample Type Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Formato 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100
Test Principle The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Ghrelin (GHRL). A competitive inhibition reaction is launched between biotin labeled Ghrelin (GHRL) and unlabeled Ghrelin (GHRL) (Standards or samples) with the
Assay procedure summary 1. Prepare all reagents, samples and standards
UniProt ID Q8AV73

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Especificidad:

This assay has high sensitivity and excellent specificity for detection of Ghrelin (GHRL).
No significant cross-reactivity or interference between Ghrelin (GHRL) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ghrelin (GHRL) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ghrelin (GHRL) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Estabilidad:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

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