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ELISA Kit for Anti-Gonadotropin Releasing Hormone Antibody (Anti-GnRH)
CC-AEA843Po
Producto nuevo
Comprando este producto generará 9 Biopuntos. Su cesta contiene un total 9 Biopuntos puede ser convertido en un Biobonos Descuento 36.00EUR.
Hoja técnica
| Size | 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100 |
| Applications | Enzyme-linked immunosorbent assay for Antibody Detection. |
| Detection Range | 3.12-200ng/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 1.15ng/mL |
| Research Area | Endocrinology |
| Organism species | Sus scrofa |
| Alternative Names | GNRH1 |
| Item Name | Gonadotropin Releasing Hormone |
| Assay Length | 2h, 30min |
| Method | Competitive Inhibition |
| Sample Type | serum |
| Test Principle | The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is |
| Assay procedure summary | 1. Prepare all reagents, samples and standards |
| UniProt ID | - |
Más información
This assay has high sensitivity and excellent specificity for detection of Anti-Gonadotropin Releasing Hormone Antibody (Anti-GnRH).
No significant cross-reactivity or interference between Anti-Gonadotropin Releasing Hormone Antibody (Anti-GnRH) and analogues was observed.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Gonadotropin Releasing Hormone Antibody (Anti-GnRH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Gonadotropin Releasing Hormone Antibody (Anti-GnRH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.