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ELISA Kit for Phosphoinositide Kinase, FYVE Finger Containing (PIKFYVE)
CC-SEL531Hu
Producto nuevo
Comprando este producto generará 7 Biopuntos. Su cesta contiene un total 7 Biopuntos puede ser convertido en un Biobonos Descuento 28.00EUR.
Hoja técnica
| Size | 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100 |
| Applications | Enzyme-linked immunosorbent assay for Antigen Detection. |
| Detection Range | 0.156-10ng/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 0.057ng/mL |
| Research Area | Enzyme & Kinase |
| Organism species | Homo sapiens (Human) |
| Alternative Names | PIP5K3 |
| Item Name | Phosphoinositide Kinase, FYVE Finger Containing |
| Assay Length | 3h |
| Method | Double-antibody Sandwich |
| Sample Type | tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Formato | 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100 |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphoinositide Kinase, FYVE Finger Containing (PIKFYVE). Standards or samples are then adde |
| Assay procedure summary | 1. Prepare all reagents, samples and standards |
| UniProt ID | Q9Y2I7 |
Más información
This assay has high sensitivity and excellent specificity for detection of Phosphoinositide Kinase, FYVE Finger Containing (PIKFYVE).
No significant cross-reactivity or interference between Phosphoinositide Kinase, FYVE Finger Containing (PIKFYVE) and analogues was observed.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phosphoinositide Kinase, FYVE Finger Containing (PIKFYVE) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phosphoinositide Kinase, FYVE Finger Containing (PIKFYVE) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.