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ELISA Kit for Transforming Growth Factor Beta 1 (TGFb1)
CC-CEA124Bo
Producto nuevo
Comprando este producto generará 7 Biopuntos. Su cesta contiene un total 7 Biopuntos puede ser convertido en un Biobonos Descuento 28.00EUR.
Hoja técnica
| Size | 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100 |
| Storage Conditions | -20°C |
| Shipping Conditions | Entre 4°C y 8°C |
| Applications | Enzyme-linked immunosorbent assay for Antigen Detection. |
| Detection Range | 2.47-200pg/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 0.83pg/mL |
| Research Area | Cytokine |
| Organism species | Bos taurus |
| Alternative Names | TGF-B1 |
| Item Name | Transforming Growth Factor Beta 1 |
| Assay Length | 2h |
| Method | Competitive Inhibition |
| Sample Type | serum, platelet-poor plasma, tissue homogenates, cell culture supernates and other biological fluids |
| Formato | 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100 |
| Test Principle | This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Transforming Growth Factor Beta 1 (TGFb1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin lab |
| Assay procedure summary | 1. Prepare all reagents, samples and standards |
| UniProt ID | P18341 |
Más información
This assay has high sensitivity and excellent specificity for detection of Transforming Growth Factor Beta 1 (TGFb1).
No significant cross-reactivity or interference between Transforming Growth Factor Beta 1 (TGFb1) and analogues was observed.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transforming Growth Factor Beta 1 (TGFb1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transforming Growth Factor Beta 1 (TGFb1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.