CLIA Kit for Vitamin D2 (VD2) Ver mas grande

CLIA Kit for Vitamin D2 (VD2)

CC-CCA921Ge

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48T, 96T, 96T×5, 96T×10, 96T×100

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Comprando este producto generará 8 Biopuntos. Su cesta contiene un total 8 Biopuntos puede ser convertido en un Biobonos Descuento 32.00EUR.


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Hoja técnica

Size 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100
Storage Conditions -20°C
Shipping Conditions Entre 4°C y 8°C
Applications Chemiluminescent immunoassay for Antigen Detection.
Detection Range 1.17-300ng/mL
Sensitivity The minimum detectable dose of this kit is typically less than 0.45ng/mL
Research Area Metabolic pathway
Organism species Pan-species (General)
Alternative Names Ergocalciferol
Item Name Vitamin D2
Assay Length 2h
Method Competitive Inhibition
Sample Type Serum, plasma and other biological fluids
Formato 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100
Test Principle The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Vitamin D2 (VD2). A competitive inhibition reaction is launched between biotin labeled Vitamin D2 (VD2) and unlabeled Vitamin D2 (VD2) (Standards or samples) wi
Assay procedure summary 1. Prepare all reagents, samples and standards
UniProt ID -

Más información

Especificidad:

This assay has high sensitivity and excellent specificity for detection of Vitamin D2 (VD2).
No significant cross-reactivity or interference between Vitamin D2 (VD2) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vitamin D2 (VD2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vitamin D2 (VD2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Estabilidad:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Click here for manual in PDF

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