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CLIA Kit for Apelin 12 (AP12)
CC-CCA856Hu
Producto nuevo
Comprando este producto generará 8 Biopuntos. Su cesta contiene un total 8 Biopuntos puede ser convertido en un Biobonos Descuento 32.00EUR.
Hoja técnica
| Size | 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100 |
| Storage Conditions | -20°C |
| Shipping Conditions | Entre 4°C y 8°C |
| Applications | Chemiluminescent immunoassay for Antigen Detection. |
| Detection Range | 62.5-16,000pg/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 22.8pg/mL |
| Research Area | Metabolic pathway |
| Organism species | Homo sapiens (Human) |
| Alternative Names | APLN12 |
| Item Name | Apelin 12 |
| Assay Length | 2h |
| Method | Competitive Inhibition |
| Sample Type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Formato | 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100 |
| Test Principle | The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Apelin 12 (AP12). A competitive inhibition reaction is launched between biotin labeled Apelin 12 (AP12) and unlabeled Apelin 12 (AP12) (Standards or samples) wi |
| Assay procedure summary | 1. Prepare all reagents, samples and standards |
| UniProt ID | Q9ULZ1 |
Más información
This assay has high sensitivity and excellent specificity for detection of Apelin 12 (AP12).
No significant cross-reactivity or interference between Apelin 12 (AP12) and analogues was observed.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apelin 12 (AP12) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apelin 12 (AP12) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.