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Instant ELISA Kit for Interleukin 10 (IL10)
CC-IEA056Mu
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| Size | 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100 |
| Applications | Enzyme-linked immunosorbent assay for Antigen Detection. |
| Detection Range | 7.8-500pg/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 3.1pg/mL |
| Research Area | Cytokine |
| Organism species | Mus musculus (Mouse) |
| Alternative Names | CSIF |
| Item Name | Interleukin 10 |
| Assay Length | 1h, 10min |
| Method | Double-antibody Sandwich |
| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Test Principle | The test principle applied in this kit is enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Instant Interleukin 10 (IL10). Standards or samples and HRP-labeled detection antibody specific to Ins |
| Assay procedure summary | 1. Prepare all reagents, samples and standards |
| UniProt ID | P18893 |
Más información
This assay has high sensitivity and excellent specificity for detection of Instant Interleukin 10 (IL10).
No significant cross-reactivity or interference between Instant Interleukin 10 (IL10) and analogues was observed.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Instant Interleukin 10 (IL10) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Instant Interleukin 10 (IL10) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.