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Instant ELISA Kit for High Mobility Group Protein 1 (HMGB1)
CC-IEA399Hu
Producto nuevo
Comprando este producto generará 7 Biopuntos. Su cesta contiene un total 7 Biopuntos puede ser convertido en un Biobonos Descuento 28.00EUR.
Hoja técnica
| Size | 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100 |
| Storage Conditions | -20°C |
| Shipping Conditions | Entre 4°C y 8°C |
| Applications | Enzyme-linked immunosorbent assay for Antigen Detection. |
| Detection Range | 62.5-4,000pg/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 22.5pg/mL |
| Research Area | Infection immunity |
| Organism species | Homo sapiens (Human) |
| Alternative Names | HMG1 |
| Item Name | High Mobility Group Protein 1 |
| Assay Length | 1h, 10min |
| Method | Double-antibody Sandwich |
| Sample Type | serum, plasma and other biological fluids |
| Formato | 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100 |
| Test Principle | The test principle applied in this kit is enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Instant High Mobility Group Protein 1 (HMGB1). Standards or samples and HRP-labeled detection antibody |
| Assay procedure summary | 1. Prepare all reagents, samples and standards |
| UniProt ID | P09429 |
Más información
This assay has high sensitivity and excellent specificity for detection of Instant High Mobility Group Protein 1 (HMGB1).
No significant cross-reactivity or interference between Instant High Mobility Group Protein 1 (HMGB1) and analogues was observed.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Instant High Mobility Group Protein 1 (HMGB1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Instant High Mobility Group Protein 1 (HMGB1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.