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Wide-range ELISA Kit for Fibroblast Growth Factor 21 (FGF21)
CC-WEC918Ra
Producto nuevo
Comprando este producto generará 7 Biopuntos. Su cesta contiene un total 7 Biopuntos puede ser convertido en un Biobonos Descuento 28.00EUR.
Hoja técnica
| Size | 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100 |
| Storage Conditions | -20°C |
| Shipping Conditions | Entre 4°C y 8°C |
| Applications | Enzyme-linked immunosorbent assay for Antigen Detection. |
| Detection Range | 12.35-1,000pg/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 4.77pg/mL |
| Research Area | Cytokine |
| Organism species | Rattus norvegicus (Rat) |
| Item Name | Fibroblast Growth Factor 21 |
| Assay Length | 3h |
| Method | Competitive Inhibition |
| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Formato | 48T, <i>96T</i>, 96T×5, 96T×10, 96T×100 |
| Test Principle | This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Wide-range Fibroblast Growth Factor 21 (FGF21) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between bioti |
| Assay procedure summary | 1. Prepare all reagents, samples and standards |
| UniProt ID | Q8VI80 |
Más información
This assay has high sensitivity and excellent specificity for detection of Wide-range Fibroblast Growth Factor 21 (FGF21).
No significant cross-reactivity or interference between Wide-range Fibroblast Growth Factor 21 (FGF21) and analogues was observed.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range Fibroblast Growth Factor 21 (FGF21) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Fibroblast Growth Factor 21 (FGF21) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.