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Vaccinia Virus Capping Enzyme
AB-P9373
Producto nuevo
Vaccinia Virus Capping Enzyme
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Hoja técnica
| Size | 50 uL |
| Storage Conditions | Store at -20ºC or lower. Aliquot to avoid repeated freezing and thawing. |
| Supplied Product | 10x Capping Buffer |
| Form | Liquid |
| Recomended Dilution | In Vitro Transcription<br>The optimal working dilution should be determined by the end user. |
| Storage Buffer | 20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 1 mM DTT, 0.1mM EDTA, 0.1% Triton X-100, 50% glycerol. |
Más información
Vaccinia virus capping enzyme is derived from a recombinant E.coli strain carries the genes for the Vaccinia capping enzyme. This single enzyme is composed of two subunits (D1 and D12) and has three enzymatic activities (RNA triphosphatase and guanylyl transferase by the D1 subunit and guanine methyltransferase by the D12 subunit). Vaccinia virus Capping Enzyme is effective to catalyze the formation of cap structure, which can specifically attach the 7-methylguanylate cap structure (m7Gppp, Cap 0) to the 5' end of RNA. Cap structure (Cap 0) plays an important role in mRNA stabilization, transport, and translation in eukaryotes. Capping RNA by the enzymatic reaction is an effective and simple method which can significantly improve the stability and translation of RNA for in vitro transcription, transfection, and microinjection.