This kit can recover high-purity DNA fragments containing no salt or low salt concentration and no impurities such as protein and RNA from various concentrations of agarose gel. The 200 bp-10 kb DNA fragment has a recovery rate of 80% or more. and single-stranded. double-stranded DNA fragments and circular plasmid DNA can be recovered. The recovered fragment DNA can be directly subjected to enzyme digestion,enzyme ligation,and sequencing reaction.